Journal: Frontiers in Pharmacology
Article Title: Chronic Allergen Challenge Induces Corticosteroid Insensitivity With Persistent Airway Remodeling and Type 2 Inflammation
doi: 10.3389/fphar.2022.855247
Figure Lengend Snippet: IL-4 + Th2 cell populations persist in MA-challenged mice. (A) OVA- and (E) MA-challenged mice exhibited significant increases in Th2 cell populations. (A) Treatment with 1 and 3 mg/kg reduced Th2 cell populations in OVA-challenged mice. (B) BAL levels of IL-4 were not significantly increased in OVA-challenged mice. (C) BAL levels of IL-13 were significantly increased in OVA-challenged mice and reduced with corticosteroid treatment. (D) Fluticasone reduces CCL17 levels in OVA mice. (E) Treatment of 1 or 3 mg/kg FP did not reduce Th2 cell populations in MA-challenged mice. (F) IL-4 or (G) IL-13 levels trended to be increased in MA-challenged mice. (H) CCL17 levels remain increased MA treated mice administered fluticasone. Data are presented as ± SEM, n = 4–10 mice per group. p < 0.05. * indicates significant difference between groups.
Article Snippet: IL-13 levels were measured in cell-free BAL supernatant using Quantikine mouse IL-13 and CCL17 ELISA kits (cat no. M1300CB and MCC170, R&D Systems, Minneapolis, MN) according to manufacturer’s instructions.
Techniques: